results slow and fast-growing groups of the mycobacterium strains were clearly differentiated based on the constructed tree of 56 common mycobacterium isolates. each species with a unique title in the tree was identified; in total, 13 nods with a bootstrap value of over 50% were supported. among the slow-growing group was mycobacterium kansasii, with m. tuberculosis in a cluster with a bootstra...

ABSTRACTTuberculosis is an infectious disease which occurs widely in the world and becomes one of the top 10 causes of death worldwide. Mutations of the RpoB gene cause the resistance of Mycobacterium tuberculosis to rifampicin that contributes to the occurrence of MDR-TB. This study aimed to determine the pattern of rpoB gene polymorphisms in the MDR M. tuberculosis in Semarang, Indonesia. Mos...

objective: the rifampicin resistance and susceptibility of mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpob gene encoding the b-subunit of rna polymerase. methods: isoniazid resistance of m. tuberculosis is related to mutations in inha , oxyr and ahpc genes which 30 to 90 percent of isoniazid resistance is occurred in 3015 codons of katg gene. the rpob a...

Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of kat...

Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of kat...

several reports have indicated that infection with non-tuberculosis mycobacteria (ntm) is increasing worldwide.therefore,monitoring species causing micobacterial infection in any region is of great importance. this study was going to detect, differentiate, and identify pathogenic mycobacteria in primary clinical samples. eighty samples collected from tuberculosis suspected patients in isfahan/i...

the aim of this study was to investigate the frequency, location and type of rpob gene mutations in mycobacterium tuberculosis (mtb) collected from patients in the southern endemic region of iran. drug susceptibility testing was determined by using the bactec system and the center for diseases control’s (cdc) standard conventional proportional method. in 29 rifampicin-resistant mtb (85%) isolat...

The rpoB gene coding for a beta-like subunit of the chloroplast DNA-dependent RNA polymerase has been located on the chloroplast genome of Euglena gracilis distal to the rrnC ribosomal RNA operon. We have determined 5760 base-pairs of DNA sequence, including 97 bp of the 5S rRNA gene, an intergenic spacer of 1264 bp, the rpoB gene of 4249 bp, 84 bp spacer and 67 bp of the rpoC1 gene. The rpoB g...

although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this stu...

Three types of phenotypic expression of β-lactam resistance have been reported in methicillin-resistant Staphylococcus aureus (MRSA): heterogeneous, homogeneous, and Eagle-type resistance. Heterogeneous-to-homogeneous conversion of β-lactam resistance is postulated to be caused by a chromosomal mutation (chr*) in addition to the expression of the mecA gene. Eagle-type resistance is a unique phe...